Quick Isolation and Purification of Extracellular Polysaccharides from Agaricus blazei Murill and Elementary Property Analysis

Abstract

Abstract: A brief and feasible system that isolated extracellular polysaccharide from Agaricus blazei Murill ferment liquid was designed. The AKTA explorer with 100 purification system was used with DEAE-cellulose and Superdex-75 to isolate quickly. FP-1-A, a glucan-protein complex, was obtained and its recovery ratio was up to 0.58g per litre ferment liquid. Its analysed property showed that the relative molecular weight was 54812. The UV scan revealed protein characteristic absorbanse apex by and the IR spectrum revealed polysaccharide absorbance apex, and the gas chromatography showed that the components of FP-1-A were composed of mannose and xylose.

Key words: Agaricus blazei Murill, glucan-protein complex, isolation, purification

ZOU Xiang. Quick Isolation and Purification of Extracellular Polysaccharides from Agaricus blazei Murill and Elementary Property Analysis[J]. FOOD SCIENCE, 2005, 26(4): 75-79.

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Quick Isolation and Purification of Extracellular Polysaccharides from Agaricus blazei Murill and Elementary Property Analysis

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