FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (10): 348-351.

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Specific Heat Capacity Assay of Mouse β-actingene PCR System Using DSC

 ZHOU  Guo-Yan, CHEN  Wei-Shi, RAN  Shu, LIN  Shu-律, BU  Jian-Ming, HUA  Ze-Zhao   

  1. Institute of Cryomedicine and Food Refrigeration, Shanghai University of Science and Technology, Shanghai 200093, China
  • Online:2007-10-15 Published:2011-11-22

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Abstract: The specific heat capacity of mouse β-actin gene system in PCR(polymerase chain reaction) was studied in this paper. By the differential scanning calorimeter (DSC), the specific heat of PCR amplified system with different PCR cycles was assayed. Further, the glass transition temperature of the PCR amplified system was also investigated in the tempera- ture range of -60～50 ℃. Experimental results showed that the more are the PCR cycles, the lower the peak value of the specific heat capacity, but the larger after 26 cycles due to the content of DNA duplexes increasing. The glass transition temperature of PCR system was -15.29 ℃。

Key words: mouse&beta, -actin gene； differential scanning calorimeter(DSC)； specific heat capacity； glass transition tem- perature；