FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (10): 398-401.

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Purification of Ice-Nucleation Active Proteins of Ice- Nucleation Active Bacteria Erwinia herbicola

 CHEN  Qing-Sen, WANG  Chang-Lu, GAO  Xiu-Zhi, YAN  Ya-Li, PANG  Guang-Chang   

  1. 1.College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300222, China; 2.Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China; 3.College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; 4.Department of Food, Beijing Agricultural College, Beijing 102206, China
  • Online:2007-10-15 Published:2011-11-22

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Abstract: The reason that ice-nucleation active bacteria (INA) have ice nucleation active is the existence of ice-nucleation active proteins (INPs). It is difficult to purify the natural INPs. Erwinia herbicola 10025A was used in this research. Ice-nucleation active proteins of the 10025A can be acquired by ultrasonic treatment and gel filtration. Polymer molecular mass is approximately 150 kD according to SDS-PAGE.

Key words: ice-nucleation active (INA) bacteria, INPs, purification of the INPs, mensuration of molecular mass