FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (11): 506-508.

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Assay Study on Total Flavanoids in Chabiaozi(Ribes mandshuricum Komarov)

 ZHANG  Gui-Lan   

  1. College of Chemistry and Chemical Technology, Xinjiang University, Urumqi 830046, China
  • Online:2007-11-15 Published:2011-11-22

Abstract: Objective: Assay study on total flavanoids to provide scientific basis for future developing and utilizing Chabiaozi resources. Methods: The optimum extraction conditions were established. The contents of total flavonoids were assayed, with rutin as control and aluminum trichloride as colorimetric reagent by clorimetric assay at 420 nm. By orthogonal design test, the factors effect levels were ascertained. The reclaim coefficient was determined. Result: The optimum extraction conditions are: 2 h and twice with 70% ethanol in extraction volume 100 ml. The optimum conditions are: addition amount 1.5ml AlCl3, heating for 40 min at 50℃. The average reclaim coefficient is 98.76%.

Key words: Chabiaozi, total flavonoids, orthogonal design test, determination