Abstract: ACE inhibitory peptides were prepared from whey protein by composite enzyme (alcalase and trypsin), and effects of peptides on blood pressure of the spontaneously hypertensive rats and angiotensin I converting enzyme(ACE) active were studied in this paper. Results showed that the peptides concentrations were 40, 80 and 160 mg/kg in vivo could depress blood pressure to 172±8, 165±7, 162±9 mmHg and the lowest number is -36±9 mmHg after administration 2 hours. At the same time ACE activity decreased with 85.62±5.23, 75.52±7.35, 61.46±8.74 U/μl(p<0.05). These results testified that the peptides had apparent effects on depressing blood pressure.

Key words: whey protein, ACE inhibitory peptide, SHR, ACE activity