Abstract:

In this study, phospholipid concentrate was extracted with n-hexane and ethanol to remove inorganic impurities and saccharides. The n-hexane extraction conditions are n-hexane amount 1.5 ml/g and standing time 1.5 h. The optimized ethanol extraction process through orthogonal test is adding phospholipid concentrate into absolute ethanol at the addition amount of 1 ml/g, and then stirring the mixed system by the speed of 100 r/min at 25 ℃ for 4 min. The saccharide content, yield, inorganic impurity, acetone insoluble substance content and moisture plus volatile substance of the obtained phospholipid concentrate product under the above conditions are 1.15%, 90%, 0.05%, 62.06% and 1.0%, respectively, and its appearance is clear and transparent.

Key words: phospholipid concentrate, n-hexane, absolue ethanol, inorganic impurity, acetone insoluble substance, saccharide content