Abstract: The article studied the quantitative and qualitative impaction of operate-factors on fiber absorption capacity when volatile substance of meat essence was extracted and analysed by applying HS-SPME coupled with GC-MS. The results appeared that in the different Remain Times (RT), the impactions of each operate-factor on the absorption of volatile substance in the SPME fiber varied. Among them the extracting temperature were the predominant factor. According to the extracting temperature, the multistage operation of HS-SPME was developed, that is the operation should apply relative high extracting temperature for lowv olatilitys ubstance, anda pplyr elative lowe xtractingt emperature forh ighv olatilitys ubstance.

Key words: headspace solid phase micro-extraction (HS-SPME), extraction capacity, multistage operation