Abstract:

In alcohol extraction process of rapeseed protein concentrate (RPC), the effects of alcohol concentration, extraction temperature, extraction times and ratio of solid to liquid on the protein content in extract were investigated, and these parameters of extraction process were optimized through orthogonal test. The orthogonal test results indicated that, the most significant factor which affects the protein content is alcohol concentration, followed by extraction temperature, solid-liquid rate, and extraction times in turn. The best extraction condition combination is A3B2C2D2, namely alcohol concentration 70%, extraction temperature 55 ℃, ratio of solid to liquid 1:6, extraction times 5. Under these conditions, the content and the yield of protein are 60.5% and 75.7% respectively, and the removal rates of the anti-nutritional compounds such as tannin, phytic acid and glucosinolate are 90. 5% , 66.7% and 99.3% respectively.

Key words: rapeseed protein concentrate, alcohol extraction, protein content