Abstract: The relationship between stability of ginkgo single-cells and cells uniformity its was studied by cells-cloning series of ginkgo. The results indicated: sterilization of medium by filtration was the good method to promote cells growth. When suspended culture of the 3rd generation callus, adding 0.5gL hydrates of casein in medium might increase planting efficiency. The planting efficiency of single-cells cloned was 27.04%. The average growth rates of GB-11 cloning lines reached 0.55g fwg fw•d.

Key words: ginkgo, callus, single-cells clone, plating efficiency, growth rates