Effect of Pueraria Flavonid on GAT-2 Expression in the Brains of Exercised Rats

Abstract

Abstract: Aim:By genechip cDNA microarray, the biomolecular mechanism of anti-fatigue of Pueraria Flavonid was studiedin the center nerve system(CNS) of the exercise-training rats. Methods: SD rats were randomly divided into three groups: normalrest group(A), exercise-training group(B) and administered-drug group(C). B group and C group rats were trained with highintensity 7 week by treadmill. The total RNAs were isolated from the brain tissue, and were purified to mRNAs by Oligotex.The purified mRNA were reversely transcribed to cDNA with hybridization probes labeled by fluorescent-dUTP. The genechipwas scanned for the fluorescent signals and showed differences by the computer analysis. Results: The GAT-2 gene was up-regulated expression in the brain of exercise-training rats compared with of rest rats and obviously up-regulated expression in thebrain of administered-drug rats compared with of exercise-training rat. Conclusion: GAT-2 gene expression in CNS of thetraining rats is regulated by Pueraria Flavonid, and its expression has an effect on CNS fatigue and plays an important role in thestudy of CNS fatigue mechanism and screening anti-fatigue functional food as new predisposing gene.

Key words: genechip, Pueraria Flavonid, GAT-2, exercise, brain, rat

TANG Liang, XIONG Zheng-Ying, ZHANG Ying-Qi. Effect of Pueraria Flavonid on GAT-2 Expression in the Brains of Exercised Rats[J]. FOOD SCIENCE, 2004, 25(2): 171-174.

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Effect of Pueraria Flavonid on GAT-2 Expression in the Brains of Exercised Rats

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