Different De-protein Processes for Extraction of Polysaccharide from Spirulina Platensis

Abstract

Abstract: Two different de-protein processes (Enzymolysis+Sevage’s method and Trichloroacetic acid method (TCA )) wereproposed to improve the traditional process for extraction of polysaccharide from Spirulina Platensis. The production rate of crudepolysaccharides (ESPE, TSPS, SPS), the contents of polysaccharides and the remained protein ratio in the crude polysaccharides,etc, were compared in this paper. ESPS and TSPS were further purified by loading on DE-52 and Sephadex - 100 . Twopolysaccharide fractions of ESPS and three fractions of TSPS were obtained. The molecular weights of these fractions were 96,200、85800, 64300, 37400, 17400 respectively. The different efficacy of different molecular weight polysaccharides shouldbe studied further.

Key words: Spirulina Platensis polysaccharide, de-protein, purification

MA Li, QIN Xiao-Lin, LIU Xiong-Min, DENG Run-Zhen. Different De-protein Processes for Extraction of Polysaccharide from Spirulina Platensis[J]. FOOD SCIENCE, 2004, 25(6): 116-119.

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Different De-protein Processes for Extraction of Polysaccharide from Spirulina Platensis

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