Abstract: In order to choose a simple, accurate and repeatable method of determination on Agaricus blazei myceliapolysaccharides, the paper compared the results of several different methods, including phenol-sulfuric method, direct-titrationetc. Futher more, the affecting factors on phenol-sulfuric method were elucidated.

Key words: Agaricus blazei mycelia, phenol-sulfuric method, direct-titration