Abstract: A rapid approach in the genomic DNA extracting from the Xanthomonas campestris was promoted in this paper. Thedetergent SDS and CTAB were used to destroy the structure of cell membrance and the cellular nucleic acids was liberated, andthen, the proteins; were removed by using pheno, chloroform, isoamyl alcohol solution, the DNA was precipitated and dissolvedby using isoproanol and TE buffer respectively. Results showed that the DNA map of agarose electrophoresis was the same asthose generated from the UNIQ-10 and D-5010A approaches. The OD260/OD280 was in 1.80～1.90.The DNA was able to bedigested by the EcoRI, XbalI. This approach could used in the genomic DNA extracting from Xanthomonas campestris rapidly.The purity of the obtained DNA was good enough for the further digestion by restriction edonucleases, the establishment ofgenomic library and the PCR analysis.

Key words: Xanthomonas campestris genomic DNA