Abstract: Objective: To optimize ethanol extraction technology of total flavonoids from Portulaca oleraceaL.. Methods: The ethanol extraction technology of total flavonoids from Portulaca oleraceaL. was optimized by orthogonal test,and the cellulase hydrolysis conditions were optimized by quadratic regression model in four variables-orthogonal test results. Results: The optimal conditions of ethanol extraction technology are as follows: material-liquid ratio 1:30,extraction temperature 60 ℃,time 60 min,and ethanol concentration 60%. The optimal cellulase hydrolysis conditions for extracting the total flavonoids from Portulaca oleraceaL. with ethanol are as follows: cellulase amount 1.9‰,hydrolysis temperature 47 ℃,pH 7.0,and time 0.7 h. Conclusion: The cellulase-assisted method is much better than the ethanol extraction method.

Key words: Portulaca oleracea L., total flavonoids, cellulase-assisted ethanol extraction