Abstract: In this study the content lentinan from dried Lentinus edodes was measured by the anshtone-sulfutic method.Through orthogonal test,the extraction ratios of lentinan by hot water method,multi-enzymatic method,ultrasonic wave-assisted method were respectively analyzed,and what was more,the advantages and disadvantages of three extraction methods were compared.The results showed that under the optimal conditions,the extraction ratios of lentinan are 7.530% in multi-enzymatic process,3.229% in hot water process,and 4.669% in ultrasonic wave-assisted process.Therefore the best extraction process of lentinan is multi-enzymatic method,and its optimal conditions are 0.1% cellulase,0.5% pectinase,2.0% papain,and extraction temperature 80 min.

Key words: lentian, hot water extraction, multi-enzymatic extraction, ultrasonic wave-assisted extraction