Abstract: Objective:To explore the effects of enzymatic hydrolysates from casein by pepsin on IL-1β,IL-6,IL-12,IFN-γ,TNF-α,L-1ra,IL-4,IL-10 and TGF-β of rabbit serum. Methods:The hydrolysates were administered to female white rabbits by gavage,intraperitoneal injection and ear intravenous injection. At 3 h,2 h and 2 h after administration respectively,ELISA kit was used assay IL-1β,IL-6,IL-12,IFN-γ,TNF-α,IL-1ra,IL-4,IL-10 and TGF-β concentrations in rabbit serum. Results:The concentrations of IL-1β,IL-1ra,IL-12 and IFN-γ are reduced by the three administration methods,and the concentrations of IL-4,IL-6,IL-10,TNF-α and TGF-β are increased. Compared with the other two administrations,intraperitoneal injection has more significant effects,which shows that cytokine network for mucosal immune system plays an important regulatory role. From the point of drug deliveries,the effect of intraperitoneal injection is the most markable,followed by ear intravenous injection,and gavage administration has no obvious effect. Conclusion:As a whole,It was suggested that the hydrolysates play an important regulatory role for body immune function by regulating cytokines level. Among the three administration methods,the most obvious method is intraperitoneal injection. The hydrolysates are managed directly by intestinal mucosal. Intraperitoneal injection is possibly a kind of the most effective drug delivery.

Key words: enzymatic hydrolysates from casein by pepsin, rabbit cytokin