Abstract: Confirm the optimum extraction technics of polyphenol of Humulus lupulus by single factor and orthogonal test: impregnant’s (acetone) concentration 70%, the ratio of materiel and impregnant 1:25(g/ml), circumfluence temperature 45℃, time 1h and extraction quantity achieve 47.29mg/g dry Humulus lupulus at this condition. The results of anti-oxidative test showed that the polyphenol extracts from Humulus Lupulus has stronger scavenging effects on [DPPH]freedom radical (IC506.72μg/ml) than ascorbic acid (IC508.79μg/ml) and tannic acid (IC507.68μg/ml), weaker than tea polyphenols (IC505.77μg/ml); it has nearly reducing capacity (EC500.111mg/ml) as ascorbic acid (EC500.101mg/ml), weaker than tannic acid (EC500.079mg/ml) and tea polyphenols (EC500.072); it also has stronger inhibition effect on MDA produced by lipid-peroxidation in liver homogenate of small rat (IC500.179mg/ml)than tannic acid (IC500.462mg/ml), weaker than tea polyphenols (IC500.136mg/ml).

Key words: polyphenol of Humulus lupulus L, extraction technics, DPPH freedom radical, reducing capacity, lipid- peroxidation