Preparation of Pure TypeⅡCollagen from Chicken Articular Cartilage

Abstract

Abstract: This paper reported the preparation of the typeⅡcollagen from chicken articular cartilage.The typeⅡcollagen was digested by various pepsin concentrations in acid solution.By investigating viscosity changes of typeⅡcollagen,the optimal pepsin concentration and digestion time have been obtained in this process.To get the purer typeⅡcollagen,DEAE-sephalose CL 6B column chromatographer was applied and repeatedly deionized in water elution.The results suggested that the optimal digestion conditions are 1% pepsin for 24 h.The extracton rate of typeⅡcollagen by two methods are 67% and 81% respectively.Analysis results of SDS-PAGE electrophoresis,RP-HPLC,amino acid components and violet chromatography showed that the prepared typeⅡcollagen by this method is a high purity product.

Key words: type Ⅱ collagen, preparation, chicken articular cartilage

CAO Hui, XU Shi-Ying. Preparation of Pure TypeⅡCollagen from Chicken Articular Cartilage[J]. FOOD SCIENCE, 2007, 28(4): 148-152.

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Preparation of Pure TypeⅡCollagen from Chicken Articular Cartilage

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