Isolation and Biological Characterization of Pseudomonas fluorescens Bacteriophages KS461-2

Abstract

Abstract: A phage,named KS461-2,was separated from the abnormal fermentation liquid of Pseudomonas fluorescens A46 strain which produced 2-keto-D-gluconic acid.The KS461-2 was tadpole-like bacteriophage as observed by electron microscope. According to ICTV(International Committee for Taxonomy of Viruses),it belongs to the Siphoviridae family and is a virulent phage. The optimal MOI(multiplicity of infection)of KS461-2 is 0.01.Temperature and pH value had remarkable effects on the stability of phage KS461-2.One-step growth curve showed that the latent period is 30min,the burst period 135 min,and the average burst size 24 phage particles fmfected cell.SDS-PAGE profiles indicated that KS461-2 contains five structural proteins, and their molecular masses are 66.0,61.0,42.5,34.7 and 14.4kDa respectively.

Key words: 2-keto-D-gluconic acid, Pseudomonas fluorescens, phage KS461-2, isolation, biological characterization

WANG Zi-Wei, SUN Wen-Jing, WANG Hui, LIU Xiang, WANG Li, LIU Jing-Ze. Isolation and Biological Characterization of Pseudomonas fluorescens Bacteriophages KS461-2[J]. FOOD SCIENCE, 2007, 28(4): 187-191.

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Isolation and Biological Characterization of Pseudomonas fluorescens Bacteriophages KS461-2

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