Abstract: In present study, the genomic DNA from the Microbe in the contaminative food was extracted directly. Firstly, the contaminative food was washed using PBS. After centrifugation, the superior liquid was extracted in extraction buffer whichcontaining SDS. The results showed that extracted genomic DNA was about 23kb. Using primers P1 and P2, the 180bp fragment of 16SrDNA gene could be amplificated. Then, the PCR productions were cloned into T-vector. A positive clone was sequenced, which was similar to the sequence of the KVD-1921-15 stain from Bacteria.

Key words: genomic DNA, microbe, extraction, method