Abstract: To study an effective and fast method of detection for listeria spedes in food.The combined Vidas and API listeria method was used to detect the presence of listeria species and to diifferentia listeria species in fod samples .The results showed that the method was applicable to all species in listeria.Depending on this measure, it was shown that the whole detection time reduce to from 10d to 5d, and the negative result was obtained within 48h (24h shorter than conventional method. It was believed that this modified method was quickes and more effective than the separate method. Satisfactory results were obtained by using this modified measure in 115 actual samples.