FOOD SCIENCE ›› 2000, Vol. 21 ›› Issue (5): 53-54.

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HPLC Analysis of Phospholipids Compositions in Soya Phospholipids

 YANG  Ling, HE  Xin-Xia, YE  Ying-Qiu, LI  Xiao-Yun, MIAO  Bei-Lei, MA  Fei-Xue   

  • Online:2000-05-15 Published:2011-11-30

Abstract: This paper dealt with qualitative and quantiative analysis of the compositions of the soybean phospholipids capsule by high performance liquid chromatography (HPLC) .We adopted Waters HPLC system using a silica column (3.9mm×300mm) .Gradient elution was carried out with isopropanol hexance (4/3 1 v/v) and isopropanal-hexance-water (8/ 6/1. 5, v/v/v) By this method the compositions of soybean phospholipids in soybean phospholipids capsule could be separated completely and the composition defined qualitatively according to the retention time. The relative content of each composition could be determined according to the peak area, and the absolute content of the predominant compositions also determined according to the content of each phospholipid in the standard sample.

Key words: HPLC Soybean phospholipids capsule Phospholipid compositions