Abstract: In submerged cultures,the aminoacylase activities of Aspergillus oryzae 3042 in vivo or in vitro were discussed,and optimal pH and temperature of pellet aminoacylase activity studied. The results showed that, Aspergillus oryzae3042 could from pellet in flask at speed 90~120rpm during submerged cultures. Aminoacylase of Aspergillus oryzae was believed to be a cell bound enzyme, which could exist in the cell or on the cel1 walI. Optimal pH and temperaure of pellet aminoacylase activity were 7.0 and 5.5, respectively

Key words: Aspergillus oryzae3042 Submerged culture Aminoacylase Pelleted form Cell bound enzyme