FOOD SCIENCE ›› 2000, Vol. 21 ›› Issue (10): 46-48.

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Study on Crocin Determination by High-Performance Liuid Chromatography

 ZHANG  De-Quan, LV Fei-Jie , TAI  Jian-Xiang   

  • Online:2000-10-15 Published:2011-12-02

Abstract: The High-performance liquid chromatographic determination of crocin was described.Crocin was separated verywell on Tuner Packing Kromasil NH2 column by the use of acetonitrile:water (80:20) as mobile phase-under the flow rate of 0.8ml/min.It was detected at 440nm by UV-VIS spectrophtometer.the retention time was 12. 19min the detection limitwas 12.5ng.The average recovery rate was 99.34%,with a good stability and reproducibility,.