Studies on Extraction Methods of C21Steroidal Glycosides

Abstract

Abstract: In order to establish the quantitative determination method for C21 steroidal glycosides in Asclepiadaceae plants and homologous health food,the extraction methods of C21 steroidal glycosides were investigated fist in this paper.The reaction of 2-desoxy sugar for cardiac glycosides’quantitative determination by thin layer chromatogaphy was used as a relative quantitative determination method for the comparison of all kinds of extraction methods.Accorfing to the structure of C21 steroidal glycosides,11 kinds of extractes were selected to do extraction experiments.The effects of other factors Such as mesh size of the material and the extraction time were also studied. At last the extraction method of C21 steroidal glycosides was determined.

Key words: Asclepiadaceae , C21 steroidal glycosides , Extraction method

SONG Jun-Mei, DING Xiao-Lin. Studies on Extraction Methods of C21Steroidal Glycosides[J]. FOOD SCIENCE, 2002, 23(9): 61-65.

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Studies on Extraction Methods of C21Steroidal Glycosides

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