Abstract: The optimum extracting process of total glucosides of Peaony from Radix Pueoniae Alba by aqueous method was obtained after using L9（34）orthogonal test plan.We selected three elements-water amounts,extraction hours and extraction times to observe their effects on the extraction of total glucosides of Peaony.The orthogonal test results showed that the optimum conditions were as follows:the water is 12 times of the Radix Puconiae Alba,it is decocted 3 times and each time is sustained for 3 hours.The major factor affecting the content of total glucosides is extraction times.On the basis of these experiments,we used D101macroreticuler resin to purify the extraction and the final content of total glucosides is up to 80.5%.

Key words: TGP(total glucosides of paeony), paeoniflorin, extraction, purification, HPLC