FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (6): 175-179.

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Extraction and Stability Study on Red Speckled Kidney Bean Pigment

CHEN  Yang, WANG  Jun-Hua, CHEN  Ya-Guang, TENG  Li-Rong, CHENG  Ying-Kun   

  1. 1.Marine College, Shandong University at Weihai, Weihai 264209, China; 2.School of Pharmacy, Shandong University, Jinan 250012, China; 3.College of Life Science, Jilin University, Changchun 130012, China
  • Online:2007-06-15 Published:2011-12-31

Abstract: The optimal extractive process and stability of the pigment extracted from red speckled kidney bean (Phaseolus vulgaris L.) were studied. Results showed that the optimum extraction yield can be obtained by 75% acidic ethanol heated up at 60 ℃ for 90 minutes with a material-solvent ratio of 1:75(g/ml). The pigment holds good resistance to light and heat. Also, the pigment is stable in acidic conditions, but sediment emerges when pH is equal to or above 4. It is also resistant to carbohydrates. Potassium sorbate, sodium benzoate and citric acid have no adverse effects on the pigment but vitamin C and H2O2 do. The pigment is relatively stable with Vitamin C of lower concentration. Metallic ions Na+, K+ and Ca2+ have no effect on the pigment, but Fe3+, Cu2+, Fe2+, Zn2+ and Mg2+ have apparent effects on it.

Key words: red speckled kidney bean, natural pigment, extraction, stability