Abstract: Casein was hydrolyzed with alcalase to prepare phospopeptides(CPPs)in a batch stirred tank reactor(BSTR).The effecfs of operating parameters－ temperature,pH,enzyme concentration,substrate concentration and time－ on the degree of hydrolysis were studied by the pH－ stat technique.The optimal operating conditions(40℃， pH10,0.2％ enzyme,2.5g/L casein and 30min) were obtained.A correlation was also established in terms of the multiple linear regression.

Key words: Casein alcalase phospopeptides degree of hydrolysis operating parameters