Abstract: Studied the properties of CPPs derived from different degrees of hydrolysis of casein and found the relationship between the CPPs activity to prevent calcium precipitate and the degree of hydrolysis of casein.The result of study showed that CPPs contained different N/P at the different ratios of enzyme to substrate.The activity of CPPs to prevent calcium precipitate was different at different N/P.The activity of CPPs to prevent calcium precipitate was lower as N/P was higher,and was higher as N/P was lower.

Key words: Degree of hydrolsis of casein Casein Phosphopeptides (CPPs) Trypsin preventing calcium precipitate