FOOD SCIENCE ›› 2001, Vol. 22 ›› Issue (5): 52-56.
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KOU Yun-Tong, MA Hong-Ming, LIU Chen-Guang
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Abstract: We established a rapid,sensitive and specific polymerase chain reaction(PCR)method for detection of Listeria monocytogenes(LM)in food.The selected primer could be amplified by PCR showing excellent features of LM. detection limit for LM in artificially contaminated food was 4－8 colony forming units(CFU)per 10g of food;enrichment limit was 7.2－11×103/ml;PCR reaction system imit was 86－132 CFU.400 naturally contaminated food samples were detected by both methods,and showed completely identical results.
Key words: PCR Listeria monocytogenes Food Detection
KOU Yun-Tong, MA Hong-Ming, LIU Chen-Guang. PCR Rapid Detection of Listeria Monocytogenes in Food[J]. FOOD SCIENCE, 2001, 22(5): 52-56.
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