Abstract: A simple, rapid and accurate quantitative method was established for the determination of puerarin and daidzin in Pueraria Lobata (Wild) by high performance liquid chromatography. The total isoflavone was extracted in P.lobata with 70％ EtOH solution by microwave oven for 2min. Separation was obtained by using Symmetry C 18 column .The retention time for puerarin and daidzin was 4.38min. and 8.15min respectively.The calibration curve was linear in the range of 0.115～ 1.38μ g/ml with r=0.9991 for puerarin and 0.12～ 1.44μ g/ml with r=0.9998 for daidzin. The average recovery rate was 103.31％ with CV=1.37％ for puerarin and 98. 73％ with CV=2. 43％ for daindzin.

Key words: Pueraria Lobata (Wild) Ohwi Puerarin Daidzin High－ performance liquid chromatography