Optimization the extraction technology of total polyphenol from propolis

Abstract

Abstract: Central composite design and response surface ((CCD-RSM) was employed to optimize the ultrasonic extraction conditions of total polyphenol from propolis . Based on single factor experiments, the ultrasonic time, liquid material ratio and ethanol concentration were investigated.With Folin-Ciocalteus determining the total polyphenol , the value was analied to determine the best extraction conditions. The results showed that the optimal extraction conditions :the extraction time, ethanol concentration and solid-to-liquid ratio were 24.0 min, 77.0%and1:14 (g/mL), respectively. the experimental average value is 3.34% (n = 3 ,R=0.14%) , the total polyphenol content of propolis was 2.86% from best experimental conditions of single factor,better than the results of single factor.This method is simple, rapid and effective, and improve the extraction of polyphenols in propolis

CLC Number:

TS255.36

References

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