Abstract: In order to establish an enzymatic method for collagen extraction from egg shell membrane, the influence of enzyme type, pH, enzyme dose, material-to-water ratio and extraction time on extraction efficiency was investigated and these extraction parameters were optimized one-factor-at-a-time experiments combined with an L9(34) orthogonal array design. The optimal extraction condition was hydrolysis for 5 h with 6% papain at normal temperature and a material-to-water ratio of 1:100 (g/mL), resulting in an extraction rate of 0.91%. As a result, an efficient extraction method was obtained. Collagen extraction from egg shell membrane by this method not only avoids environmental pollution caused by abandoned egg shell membrane, but also has great practical value in the collagen production industry.

Key words: egg shell membrane, collagen, enzymolysis, extraction