Optimization of cellulase enzymolysis on extraction by response surface methodolog

Abstract

Abstract: To discuss the impact of cellulase on extraction of resveratrol，response surface methodology(RSM) was investigated with the content of resveratrol as the response value and cellulase dosage, enzymolysis time and enzymolysis temperature as indepengdent variables, on basis of single factor experimengts,.The results showed that cellulase dosage had a extremely significant effect on the yield of resveratrol, but enzymolysis time and enzymolysis temperature had no significant effect. The effects order of three factors on content of resveratrol were as follows: cellulase dosage first, enzymolysis time second, and then enzymolysis temperature. The optimum enzymolysis conditions were dosage of cellulase 1.30%, time of enzymolysis 1.48h and temperature of enzymolysis 55.49℃. Under optimal conditions, the content of resveratrol was finally determined to be 0.746 mg.g-1, which was 1.15 times of that of non-optimization.

CLC Number:

TS255
S663

Shi-Quan QIAN. Optimization of cellulase enzymolysis on extraction by response surface methodolog[J]. FOOD SCIENCE, 2013, 34(4): 75-79.

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