Abstract:

A method to isolate and purify gulcoraphanin (4-methylsufinylbutyl glucosionlate) from broccoli seeds was
reported. Gulcoraphanin was extracted from broccoli seeds with a mixture of methanol and water, and purified by DEAESephadex
A25 anion exchange column chromatography. Pure glucoraphanin was obtained by preparative HPLC, and
identified by HPLC-MS, NMR, IR and UV spectroscopy. The results were consistent with the molecular structure of
glucoraphanin. The described method is rapid, robust and suitable for preparing pure glucoraphanin.

Key words: glucoraphanin, broccoli seeds, extraction, isolation, identification