Anti-Alcohol Function of Corn Peptides Produced by Continuous Enzymatic Membrane Reaction

Abstract

Abstract: The purpose of this study was to comparatively assess the anti-alcohol function of corn peptides (CPs) produced by continuous or intermittent enzymatic membrane reaction (EMR) in animals. Righting reflex and anti-alcohol tests were carried out with mouse models and Haiwangjinzun (HWJZ), a commercial anti-alcohol drug in China, was used as a positive control to observe ebriety rate and ebriety time. Besides, serum alcohol level and hepatic alcohol dehydrogenase (ADH) activity were measured. The results showed that CPs produced by continuous EMR could highly significantly shorten the ebriety time of mice, decrease the level of alcohol in the serum, and activate the activity of ADH in the liver (P ＜0.01). Taken together, our results demonstrate that CPs has an excellent anti-alcohol function. Furthermore, the anti-alcohol function of CPs produced by continuous EMR is superior to that of those produced by intermittent EMR. CPs administration shortly after alcohol consumption is more effective. The anti-alcohol function of CPs produced by continuous EMR at a dose of 300 mg/kg is comparable to that of HWJZ at 450 mg/kg. Thus, CPs produced from continuous EMR can be considered as an excellent anti-alcohol drug.

Key words: corn peptides (CPs), anti-alcohol function, continuous process

CLC Number:

TS253.9

References

[1] Wojciech J, Maciej S. Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the cancer diseases[J]. Clinica Chimica Acta, 2008, 395: 1-5.
[2] 孙冀平, 沈蓓英. 蛋白肽饮料的醒酒机理和制备工艺的讨论[J]. 食品与发酵工业, 1999, 25(4):64-66.
[3] 白雪松, 韩春姬, 李克. 复方轮叶党参的醒酒作用实验研究[J]. 延边大学医学学报, 2004, 27 (3):166-169.
[4] Huang Wenhao, Sun Jie, He Hui, et al. Antihypertensive effect of corn peptides, produced by a continuous production in enzymatic membrane reactor, in spontaneously hypertensive rats[J]. Food Chemistry, 2011, 128:968-973.
[5] 李升福, 闻海波. 玉米蛋白水解物的功能性—抗疲劳的研究[J].食品科学, 2006, 27(08): 245-247.
[6] Li Hongmei, Hu Xin, Guo Ping, et al. Antioxidant properties and possible mode of action of corn protein peptides and zein peptides[J]. Journal of Food Biochemistry, 2010, (34): 44–60.
[7] 郭辉, 何慧, 于国才, 等. 玉米肽对免疫性肝损伤小鼠的保护作用[J]. 中国粮油学报, 2009, 24(10):41-45.
[8] Yamaguchi M, Nishikiori F, Ito M, et al. The effects of corn peptide ingestion on facilitating alcohol metabolism in healthy men[J]. Biosci Biotech. Biochem, 1997, 61(9): 1474-1481.
[9] Yamaguchi M, Nishikori F, Yoshida M, et al. Water soluble vegetable oligopeptides: comparative study on alcohol metabolism and plasma amino acid concentrations in stroke-prone spontaneously hypertensive rats[J]. Food Biochem, 1998, 22 (3): 227-244.
[10] 隋玉杰, 何慧, 王进, 等. 中性蛋白酶及碱性蛋白酶制备玉米粗肽的醒酒活性比较[J].中国粮油学报,2006,21(3):102-106.
[11] 隋玉杰, 何慧, 石燕玲, 等. 玉米肽的醒酒活性体外试验及其醒酒机理研究[J]. 中国粮油学报, 2008, 23(5): 54-58.
[12] 郭辉, 何慧, 韩樱, 等. 玉米肽对小鼠酒后肝脏乙醇脱氢酶活力的影响及醒酒机理[J]. 食品科学, 2011, 32(11): 265-269.
[13] 于国才. 新型高活性醒酒保肝玉米肽的制备、活性与结构 [D]. 武汉：华中农业大学，2010.
[14] 于国才, 何慧, 曹汝鸽, 等. 超滤法制备高活性醒酒玉米肽以及pH值对超滤的影响[J]. 中国粮油学报,2010,25(8):98-103.
[15] Madhusudhan M C, Raghavarao K S M S, Sanjay N. Integrated process for extraction and purification of alcohol dehydrogenase from Baker’s yeast involving precipitation and aqueous two phase extraction[J]. Biochem Eng J, 2008, 38(3): 414–420.
[16] 王双玉, 李坤, 阚国仕, 等. 玉米抗氧化肽的制备及其对小鼠急性酒精肝损伤的保护作用[J]. 食品研究与开发, 2007, 28(3): 29-33.