Detection of arcABC Gene Cluster in Oenococcus oeni

Abstract

Abstract: PCR and GC-MS were used to detect arcABC gene cluster in fifty O. oeni strains conserved in the College of Enology of Northwest A&F University to determine their abilities for ethyl carbamate production. All the O. oeni strains were found to possess the genes arcA, arcB and arcC, i.e. arcABC gene cluster. A trace amount of ethyl carbamate was found after 30 d of cultivation in a simulated wine culture medium fortified with arginine, indicating that all these strains have the potential to produce ethyl carbamate.

Key words: arginine deiminase pathway, Oenococcus oeni, arcABC gene cluster, PCR technique, gas chromatography-mass spectrometry (GC-MS)

CLC Number:

TS261.1

References

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