Optimization of Ultrasonic-assisted Extraction of Total Falconoids from Malus hupehensis by Response Surface Method

Abstract

Abstract:

Response surface analysis methodology (RSM) was applied to optimize the ultrasonic-assisted extraction conditions of total flavonoids from Malus hupehensis. The concentrations of ethanol in solvent, extraction time, ultrasonic power and ratio of liquid to solid were chosen as casual factors. Base on the single-factor experiments four-factor-three-levels box behnken experiments design were applied. RSM was employed to study on the effect of these factors on the yield of total flavonoids from Malus hupehensis. The results show that the optimum ultrasonic-assisted conditions were ratio of liquid to solid 50:1(mL/g), 67.35 % ethanol, extracting time 3.2 h, ultrasonic power 162.4 W, Validation experiments were carried out at these optimum conditions. The predicted value of the maximal yield of flavonoids is 12.78 %, while the effective value is 12.76 %, the relative error between actual values and predicted values is 0.02 %. These results demonstrated that the method is feasible.

CLC Number:

R 284.2

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