Determination of nitrofurazone residue in aquaculture water by HPLC

Abstract

Abstract: We develop a method for nitrofurazone residue determined in the aquaculture water by High Performance Liquid Chromatography (HPLC).The stability of nitrofurazone was analyzed for the assistance to screen the determination condition. The results show that liquid-liquid extraction wasn’t suitable for extracting the nitrofurazone in water, while the solid-phase extraction is acceptable. The efficiency of several solid-phase extraction cartridges was compared to optimize the extract condition. MCX, HLB, C18 cartridge are capable of retaining nitrofurazone, which MCX is suitable for alkaline extract, HLB efficiency is insensitive to pH value, and the C18 is less effective than HLB. The methods, recovery of 85% -95%, relative standard deviation of less than10%, detection limit of 0.25μg/L, can be applied to scrutinize the nitrofurazone residue in aquaculture water.

CLC Number:

X824

References

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