Optimization and Kinetics of Enzymatic Hydrolysis of Dioscorea esculenta (Lour) Burkill Tubers

Abstract

Abstract: The hydrolysis of Dioscorea esculenta (Lour) Burkill Tubers by thermostable alpha-amylase for starch liquefaction was optimized by response surface methodology. Meanwhile, the hydrolysis process was studied kinetically. The results showed that under the optimized hydrolysis conditions: enzyme dosage of 0.15 mL/g, hydrolysis temperature of 90 ℃, hydrolysis pH of 6.0, substrate concentration of 70 mg/mL and hydrolysis time of 50 min, the DE (dextrose equivalent) was 55.69%. The vm and Km were determined to be 1.413 mg/(mL·min) and 69.97 mg/mL, respectively.

Key words: Dioscorea esculenta (Lour) Burkill, response surface analysis, enzymatic hydrolysis, kinetics

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Optimization and Kinetics of Enzymatic Hydrolysis of Dioscorea esculenta (Lour) Burkill Tubers

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