Abstract: An HILC-MS/MS method was developed to simultaneously determine melamine and cyanuric acid in food contact packaging materials. Samples were extracted with H2O, 4% acetic acid (V/V), 10% ethanol isooctane (V/V) and olive oil separately, kept in a 70 ℃ incubator for 2 h and then filtered through a 0.2 μm microporous membrane prior to injection into an HILC-MS/MS system. Melamine and cyanuric acid were separated, identified and quantified on HILC column by gradient mobile phase and monitored in a positive/negative electrospray ionization and multiple reaction monitor (MRM) mode. A good linear range was achieved over the concentration range of 1－100 ng/mL and 2－200 ng/mL for melamine and cyanuric acid in different stimulants, respectively. The limits of detection and limits of quantification were in the range of 0.4－0.8 ng/mL. The recovery rates of melamine and cyanuric acid were 134.0%－86.2% and 156.0%－78.9% with RSDs of 6.44%－17.93% and 5.95%－11.78%. This method was simple, rapid, and accurate, and suitable for the determination of melamine and cyacuric acid in food contact packaging materials.

Key words: HILC-MS/MS, food contact packaging materials, melamine, cyanuric acid, migration