Optimization of Polysaccharide Extraction from Pumpkin by Central Composite Design/Response Surface Methodology

doi:10.7506/spkx1002-6630-201308022

Abstract

Abstract: Objective: Central composite design/response surface methodology was employed to optimize the extraction of polysaccharides from pumpkin and a suitable type of macroporous resin was identified for the purification of crude polysaccharides. Methods: A hot water extraction method was proposed for polysaccharide extraction from pumpkin. One-factor-at-a-time method and central composite design/response surface methodology were used to examine the effects of extraction conditions including solid-to-liquid ratio, time, temperature and repeated extraction as well as alcohol precipitation conditions on the extraction efficiency of polysaccharides. Purified polysaccharides were obtained through repeated alcohol precipitation and purification by macroporous resign chromatography. Results: The optimum extraction process of pumpkin polysaccharides was found to be three extraction cycles with a 36-fold volume of hot water at 84 ℃ for 3.2 h each followed by evaporation until one-third of the original volume and precipitation with a three-fold volume of alcohol. AB-8 resin was chosen to purify the crude polysaccharides through adsorption at 20 ℃ with an initial sample concentration of 2.84 mg/mL and a sample loading amount of 5 BV and desorption with 5 BV of 20% alcohol. The purified products contained over 60% polysaccharides. Conclusion: The optimized extraction process is simple and reliable. AB-8 resin is suitable for the purification of crude polysaccharides from pumpkin.

Key words: pumpkin polysaccharide, extraction, central composite design/response surface methodology, macroporous resin, purification

CLC Number:

TS255.36

CHEN Li-jiang，WANG Yong-jie，LIU Yu，GAO Fei，YAN Xi-chen. Optimization of Polysaccharide Extraction from Pumpkin by Central Composite Design/Response Surface Methodology[J]. FOOD SCIENCE, 2013, 34(8): 107-112.

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