Isolation and Purification of Antibacterial Components in Fermentation Broth of Paecilomyces militaris

Abstract

Abstract: Escherichia coli, Canidia albicans and Aspergillus niger were used as indicator strains for the bioassay-guided separation of antibacterial components from the ethyl acetate extract of fermentation broth of Paecilomyces militaris. The ethyl acetate extract was separated by silica gel column chromatography into two antibacterial fractions, Ⅴ and Ⅶ. Fraction Ⅴ was further separated by reversed-phase column chromatography to obtain a pure antibacterial compound, PMBA-1. At a concentration of 1.0 mg/mL, PMBA-1 presented an inhibition circle diameter of (16.21 ± 0.27) mm, (23.54 ± 0.35) mm and (27.33 ± 0.54) mm against Escherichia coli, Canidia albicans and Aspergillus niger, respectively. Another pure antibacterial compound, PMBA-2, was obtained from further separation of fraction Ⅶ by silica gel column chromatography. Its inhibition circle diameter at a concentration of 1.0 mg/mL was (21.84 ± 0.28), (16.33 ± 0.54) mm and (11.54 ± 0.51) mm for Escherichia coli, Canidia albicans and Aspergillus niger, respectively.

Key words: fermentation broth of Paecilomyces militaris, antibacterial components, isolation, purification

CLC Number:

Q939.9

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