FOOD SCIENCE ›› 2014, Vol. 35 ›› Issue (4): 44-49.doi: 10.7506/spkx1002-6630-201404010

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Optimization of Ultrasonic-Assisted Extraction of Ursolic Acid from Roots of Actinidia chinensis Using Response Surface Analysis

YANG Yan, REN Ya-mei*, MA Ting, ZHOU Jiu-qing, ZHA Jing, ZHANG Zhe-xue   

  1. College of Food Science and Engineering, Northwest Agriculture and Forestry University, Yangling 712100, China
  • Received:2013-02-18 Revised:2014-01-07 Online:2014-02-25 Published:2014-03-17
  • Contact: ren yamei E-mail:yameiren@yahoo.com

Abstract:

In this study, the conditions for ultrasonic-assisted extraction of ursolic acid (UA) from the roots of Actinidia
chinensis were optimized by response surface methodology. On the basis of single-factor experiments, a mathematical
regression model was established indicating the effect of ethanol concentration, solvent/solid ratio, extraction time and
temperature on the extraction yield of UA by response surface central composite design. Under the conditions of 225 W and
40 kHz, the optimum extraction conditions determined were as follows: 73.8% ethanol concentration, 7.80:1 solvent/solid
ratio (mL/g), 23.2 min and 79.1 ℃. Under these conditions, the experimental extraction yield of UA was 2.014%, which is
well matched with the predicted value, and the purity of UA in the extract was 49.7%.

Key words: Actinidia chinensis root, ursolic acid, ultrasonic-assisted extraction, response surface analysis

CLC Number: