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Analysis of Residual Deoxynivalenol and T-2 Toxin in Livestock and Poultry Products

ZOU Zhong-yi，HE Zhi-fei，LI Hong-jun，HAN Peng-fei，MENG Xiao，ZHOU Fang，LUO Li-yong   

  1. 1. College of Food Science, Southwest University, Chongqing 400715, China；
    2. Department of Logistics Management, China Maritime Police Academy, Ningbo 315801, China；
    3. Chongqing Special Food Programme and Technology Research Center, Southwest University, Chongqing 400715, China
  • Online:2013-07-25 Published:2013-08-02

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Abstract:

Objective: To analyze residual deoxynivalenol (DON) and T-2 toxin in products of livestock and poultry.
Methods: Residual DON and T-2 toxin in samples were extracted with acetonitrile, defatted with n-hexane, cleaned up on an
HLB cartridge, and analyzed by high performance liquid chromatography coupled with tandem mass spectrometry (HPLCMS/
MS) and quantified by an external standard method. Results: Residual DON was not observed in all tested samples
of pig dorsal muscle, pig liver, pig kidney, chicken breast, chicken thighs, chicken wings and meat products, but detected
in 28.75% of pig back fat, and the maximum residue was up to 0.4265 μg/kg. No residual T-2 toxin was detected in all
tested samples of pig liver, pig kidney and meat products, but detected in 8.33%–57.14% of pig dorsal muscle, pig back fat,
chicken breast, chicken thighs and chicken wings, and the maximum contents were 0.0704–0.4515 μg/kg. Conclusion: Trace
residues of DON and T-2 toxin can be detected in partial tested samples of livestock and poultry products.

Key words: products of livestock and poultry；deoxynivalenol；T-2 toxin, residue；high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)；analysis