Purification of Puerarin by Macroporous Resins

doi:10.7506/spkx1002-6630-201316018

Abstract

Abstract:

Macrosporous resin types D101, X-5, HD-101, D14, S-8, HC-D301, HD-8, D141, HPD100, HPD500, AB-8
and NKA-2 were compared for their adsorption and desorption capacities for the purification of puerarin from a crude
extract of Radix Puerariae. NKA-2 was found to have the strongest ability to adsorb puerarin with a static saturated
adsorption quantity of 184.9 mg/g and a desorption percentage of 90.86% by 50% ethanol. The optimal conditions for
purifying puerarin with NKA-2, as determined by orthogonal array design, were 18 mg/mL, 60% ethanol and 1 BV/h for
sample concentration, desorbent and desorption flow rate, respectively. Under the optimized conditions, the recovery
and purify of puerarin were 90.06% and 82.18%. The optimized process has potential practical applications for the
purification of puerarin.

Key words: macroporous resin, puerarin, purification, process parameters

LI Shi，YUE Ming-zhu，LI Yin-hua，FU Dong-he. Purification of Puerarin by Macroporous Resins[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201316018.

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Purification of Puerarin by Macroporous Resins

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