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Colormetric Determination of Metallothionein Using DTNB

WU Yun-hui，WANG Jun-kun，SUN Ji-peng，ZENG Jia-ling   

  1. 1. Xiamen Ocean Vocational College, Xiamen 361100, China；2. Third Institue of Oceanography State Oceanic Administration,Xiamen 361005, China；3. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
  • Online:2013-08-25 Published:2013-09-03

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Abstract:

A corlometric method for the determination of metallothionein (MT) was established using 5,5’-dithio bis-
(2-nitrobenzoic acid) (DTNB) as the derivatizing agent and measuring the absorbance of the colored product TNBA
at 412 nm. The results showed that a linear relationship of MT was obtained within the concentration range of 2–15 μg
and 10–150 μg. The corresponding equations were A412 nm = 0.0059C ＋ 0.0251 (R2 = 0.9942), and A412 nm = 0.0052C ＋
0.0201 (R2 = 0.9997). The average recovery was 99.94% (n = 6, RSD = 0.27%), with good precision (n = 6, RSD = 0. 7%).
The limit of detection of the method was 2 μg. In conclusion this study has developed a simple and rapid method to
determine MT.

Key words: metallothionein (MT)；5,5&rsquo, -dithio bis-(2-nitrobenzoic acid) (DTNB)；determination