Supercritical CO2 Extraction and Bioactivity of Polyphenols from Chinese Jujube Seeds

doi:10.7506/spkx1002-6630-201322013

Abstract

Abstract:

Polyphenols from Chinese jujube seeds were extracted by supercritical carbon dioxide extraction and their
DPPH radical scavenging activity and inhibitory activities against α-amylase, α-glucosidase and hyaluronidase were
evaluated. The optimal extraction conditions that provided maximum extraction efficiency were determined as 2.5 h,
35 MPa, 50 ℃ and 2 for time, pressure, temperature and extraction number, respectively. The extracted polyphenols
possessed strong DPPH radical scavenging activity and could effectively inhibit α-amylase, α-glucosidase and hyaluronidase
and the corresponding inhibition rates were 93.52%, 86.67%, 98.73% and 96.86% over the concentration
range tested with IC50 values of 6.16, 9.81, 7.29 mg/mL and 5.59 mg/mL (expressed as the weight of jujube seeds),
respectively. Correlation analysis suggested an extremely significant positive correlation between the content of
polyphenols and the activities to scavenge DPPH free radical and inhibit three enzymes in jujube seed extracts.
Interestingly, it was also found that the optimal conditions for high yield of polyphenols were not in accordance
with those obtained for high activity, suggesting that the extraction conditions pressure, temperature, duration
and number of repeats could not only affect the yield of polyphenols, but the biological activity as well.

Key words: jujube seed；polyphenol；supercritical CO2 extraction；DPPH radical；&alpha, -amylase；&alpha, -glucosidase；hyaluronidase

CLC Number:

TS255

LIU Jie-chao，ZHANG Chun-ling，LIU Hui，ZHENG Xiao-wei，WANG Si-xin，JIAO Zhong-gao. Supercritical CO2 Extraction and Bioactivity of Polyphenols from Chinese Jujube Seeds[J]. FOOD SCIENCE, 2013, 34(22): 64-69.

References

[1] [1] QUIDEAU S, DEFFIEUX D, DOUAT-CASASSUS C, et al. Plant polyphenols: chemical properties, biological activities, and synthesis [J]. Angew Chem Int Ed Engl, 2011, 50(3): 586-621.

[2] VISIOLI F, DE LA LASTRA CA, ANTRES-LACUEVA C, et al. Polyphenols and human health: a prospectus [J]. Crit Rev Food Sci Nutr, 2011, 51(6):524-46.

[3] 鲁周民, 刘坤, 闫忠心, 等. 2010. 枣果实营养成分及保健作用研究进展[J]. 园艺学报, 37(12): 2017–2024.

[4] 郝婕, 王艳辉, 董金皋. 金丝小枣多酚提取物的生理功效研究[J]. 中国食品学报, 2008, 8(5): 22-27.

[5] 郝会芳, 王艳辉, 苗笑阳, 等. 枣核中多酚物质提取条件的初步研究[J]. 华北农学报,2007,22(增刊):48-52

[6] 张吉祥，欧来良. 正交试验法优化超声提取枣核总黄酮[J]. 食品科学, 2012, 33 (4): 18-21.

[7] 张吉祥，欧来良. 枣核总黄酮的微波辅助提取工艺优化[J]. 食品科学, 2012, 33 (16): 45-49.

[8] 张志国, 陈锦屏, 邵秀芝, 等. 红枣核类黄酮清除DPPH自由基活性研究[J].食品科学, 2007, 28 (2): 67-70.

[9] GHAFOOR K, AL-JUHAIMI FY, CHOI YH. Supercritical fluid extraction of phenolic compounds and antioxidants from grape (Vitis labrusca B.) seeds [J]. Plant Foods Hum Nutr, 2012, 67(4): 407-414.

[10] SANTOS SAO, VILLAVERDE, JJ, SILVA, CM, et al. Supercritical fluid extraction of phenolic compounds from Eucalyptus globulus Labill bark [J]. J Supercritical Fluids, 2012, 71(1):71-79.

[11] 王华. 利用超临界技术萃取石榴叶总酚工艺研究[J]. 食品科学, 2009, 30 (18 ): 152-154.

[12] 李军, 冯耀声. 超临界二氧化碳萃取茶多酚的研究[J]. 天然产物研究与开发, 1996, 8(3): 42-47.

[13] 石碧, 狄莹. 植物多酚[M]. 北京: 科学出版社, 2000. 19-20.

[14] SHIMADA K, FUJIKAWA K, YAHARA K, et al. Antioxidative properties of xanthin on autoxidation of soybean oil in cyclodextrin emulsion [J]. J Agric Food Chem, 1992, 40: 945–948.

[15] 焦中高, 刘杰超, 王思新, 等. 羧甲基化红枣多糖制备及其活性[J]. 食品科学, 2011, 32(17): 176-180.

[16] 包怡红, 李文星, 齐君君, 等. 提取条件对蓝靛果花色苷抗氧化活性的影响[J]. 食品科学, 2010, 31(22): 20-24.

[17] 翟梅枝, 贾彩霞, 景炳年, 等. 不同提取条件对核桃叶中抗病毒物质活性的影响[J]. 林业科学, 2007, 43(7): 149-151.

[18] 刘育玲, 姚开, 贾冬英, 等. 茶多糖酶法提取的优化条件及其对葡萄糖激酶活性的影响[J]. 食品科技, 2010, 35(2):134-137

[19] 罗娅, 王小蓉, 张勇, 等. 不同提取条件对草莓果实抗氧化物质和抗氧化活性的影响[J]. 食品科学, 2011, 32(14): 108-112.

[20] TUNDIS R, LOIZZO M R, MENICHINI F. Natural products as α-amylase and α-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: An update [J]. Mini Rev Med Chem, 2010, 10: 315-331.

[21] SUQIMOTO K, IIZAWA T, HARADA H, et al. Cartilage degradation independent of MMP/aggrecanases [J]. Osteoarthritis Cartilage, 2004, 12(12): 1006–1014.