Screening of High-yield DHA-producing Crypthecodium cohnii Mutant

doi:10.7506/spkx1002-6630-201317049

FOOD SCIENCE

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Screening of High-yield DHA-producing Crypthecodium cohnii Mutant

SHE Jun1,2，TIAN Hua1，CHEN Tao3，HE Dong-ping1,2,*

1. College of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China；2. Hubei Pashun Foods
Co. Ltd., Wuhan 430040, China；3. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

Online:2013-09-15 Published:2013-09-27
Contact: HE Dong-ping

Abstract

Abstract:

The original strain C. cohnii ATCC 30772 was irradiated by 60Co-γ ray. Mutant 2.4k-2A2-5 was selected as a
desired positive mutant according to its growth and development, Sudan black B staining, biomass, oil content and DHA
content, following screening and rescreening. The average DHA production was 39.04%, which was 3.39% higher than the
original strain. The yield and content of oil produced by this mutant was 51.66% and 63.37%, respectively, which were also
higher than those by the original strain. The DHA yield (measured in 7.14 g/L fermented liquid) was increased by 56.92%
compared to that produced by the original strain.

Key words: Crypthecodium cohnii；60Co-&gamma, rays；DHA

CLC Number:

TS222.1

SHE Jun1,2，TIAN Hua1，CHEN Tao3，HE Dong-ping1,2,*. Screening of High-yield DHA-producing Crypthecodium cohnii Mutant[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201317049.

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Screening of High-yield DHA-producing Crypthecodium cohnii Mutant

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