Abstract:

Polypehenols from blueberry pomace, a byproduct of blueberry juice production, were purified by macroporous
resin chromatography and HPLC-DAD-MS was used to analyze the polyphenol composition of extracts. The results
indicated that the absorption capacity and desorption capacity of AB-8 resin were 24.38 mg/g and 95.49%, respectively. In
addition, the time for reaching the adsorption and desorption equilibrium in AB-8 resin was short, so it was the most suitable
to purify blueberry pomace polyphenols. The optimum adsorption parameters of AB-8 resin were polyphenol concentration
of 2.6 mg/mL, pH 2, feeding rate of 2.0 mL/min, and the optimal desorption parameters were ethanol concentration of 60%
and elution rate of 2.0 mL/min. After purification, the purity of polyphenols was increased from 11.05% to 59.29%. HPLCDAD-
MS analysis showed that the major components of blueberry pomace polyphenols included 7 kinds of anthocyanins
such as delphindin-3-galactoside, delphindin-3-glucoside, malvidin-3-glucoside, petunidin-3-galactoside, petunidin-3-
glucoside, malvidin-3-galactoside, and cyanidin-3-galactioside.

Key words: blueberry pomace polyphenols, macroporous resin, purification, high-performance liquid chromatographydiode-array detection-mass spectrometry (HPLC-DAD-MS)